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nextflex rapid directional rna seq library prep kit  (Revvity)


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    Revvity nextflex rapid directional rna seq library prep kit
    Nextflex Rapid Directional Rna Seq Library Prep Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nextflex+rapid+directional+rna+seq+library+prep+kit/pmc10233378-67-0-8?v=Revvity
    Average 93 stars, based on 94 article reviews
    nextflex rapid directional rna seq library prep kit - by Bioz Stars, 2026-07
    93/100 stars

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    In vivo tumor growth assessment and RNA-seq characterization of murine Braf V600E melanoma cells with ptgs1 , ptgs2 , or ptges KO. A, Cell proliferation assay in scramble, ptgs1- KO, ptgs2 -KO, and ptges -KO murine Braf V600E melanoma cells. Results represent the fold change relative to the OD value of baseline levels at time 0 for each cell (0, 24, 48, and 72 hours after culture; n = 6). Differences between two groups were analyzed by repeated measures one-way ANOVA, followed by Tukey post hoc test. B, Left, Tumor volume measurement at indicated timepoints for scramble, ptgs1 -KO, ptgs2- KO, and ptges -KO cells. A total of 1 × 10 6 cells per mouse were injected subcutaneously into the flanks of C57BL/6J mice: n = 8 mice each for scramble, ptgs1- KO, and ptgs2 -KO groups, and n = 7 for ptges -KO group. Differences in tumor volume between two groups were analyzed by repeated measures one-way ANOVA, followed by Tukey post hoc test. B, Right, Tumor volumes for individual mice in each group are shown. C, Tumor-free survival (tumor volume < 1,000 mm 3 ) was estimated using the Kaplan–Meier method and compared using the log-rank test. D, Heat map showing the unsupervised hierarchical clustering of the mRNA levels (Reads Per Kilobase Million [RPKM]) of eight collagen-related genes across scramble, ptgs1 -KO, ptgs2- KO, and ptges- KO cells. RNA was extracted from three different subclones in each cell line. E, Spearman correlation values between immune-suppressive markers and collagen-related genes in melanoma samples from TCGA-SKCM dataset. Graph values represent mean ± SD. **, P < 0.01. N.S., not statistically significant.

    Journal: Cancer Research Communications

    Article Title: Inhibition of Microsomal Prostaglandin E2 Synthase Reduces Collagen Deposition in Melanoma Tumors and May Improve Immunotherapy Efficacy by Reducing T-cell Exhaustion

    doi: 10.1158/2767-9764.CRC-23-0210

    Figure Lengend Snippet: In vivo tumor growth assessment and RNA-seq characterization of murine Braf V600E melanoma cells with ptgs1 , ptgs2 , or ptges KO. A, Cell proliferation assay in scramble, ptgs1- KO, ptgs2 -KO, and ptges -KO murine Braf V600E melanoma cells. Results represent the fold change relative to the OD value of baseline levels at time 0 for each cell (0, 24, 48, and 72 hours after culture; n = 6). Differences between two groups were analyzed by repeated measures one-way ANOVA, followed by Tukey post hoc test. B, Left, Tumor volume measurement at indicated timepoints for scramble, ptgs1 -KO, ptgs2- KO, and ptges -KO cells. A total of 1 × 10 6 cells per mouse were injected subcutaneously into the flanks of C57BL/6J mice: n = 8 mice each for scramble, ptgs1- KO, and ptgs2 -KO groups, and n = 7 for ptges -KO group. Differences in tumor volume between two groups were analyzed by repeated measures one-way ANOVA, followed by Tukey post hoc test. B, Right, Tumor volumes for individual mice in each group are shown. C, Tumor-free survival (tumor volume < 1,000 mm 3 ) was estimated using the Kaplan–Meier method and compared using the log-rank test. D, Heat map showing the unsupervised hierarchical clustering of the mRNA levels (Reads Per Kilobase Million [RPKM]) of eight collagen-related genes across scramble, ptgs1 -KO, ptgs2- KO, and ptges- KO cells. RNA was extracted from three different subclones in each cell line. E, Spearman correlation values between immune-suppressive markers and collagen-related genes in melanoma samples from TCGA-SKCM dataset. Graph values represent mean ± SD. **, P < 0.01. N.S., not statistically significant.

    Article Snippet: Only RNA samples with high quality (RNA Integrity Number [RIN] > 8.0) and high purity (Optical Density [OD] 260/280 = 1.8–2.0) were used. mRNA libraries were generated with a Bioo Scientific NEXTflex Rapid Directional RNA sequencing (RNA-seq) Library Prep Kit.

    Techniques: In Vivo, RNA Sequencing, Proliferation Assay, Injection